Quantitative Analysis of Hydroxamic Acids by Paper Chromatography

Determination of monoamino monocarboxylic acids by quantitative paper chromatography.

Many methods for chromatographic separation of amino acids are now available, but their subsequent determination, generally employing ninhydrin, often leaves much to be desired. It was felt, therefore, that there was a need for a simple method of analysis based on the convenient paper technique and employing some means of determination less fickle than ninhydrin colorimetry. The method now pres...

Separation of Amino Acids by Paper Chromatography

Chromatography is a common technique for separating chemical substances. The prefix “chroma,” which suggests “color,” comes from the fact that some of the earliest applications of chromatography were to separate components of the green pigment, chlorophyll. You may have already used this method to separate the colored components in ink. In this experiment you will use chromatography to separate...

Quantitative Analysis of Fatty Acids by Gas-Liquid Chromatography

E. C. HORNING, Baylor University College of Medicine, Houston, Teras (Chairman) E. H. AHRENS, JR., The Rockefeller Institute, New York, New York S. R. LIPSKY, Yale University School of Medicine, New Haven, Connecticut F. H. MATTSON, Procter and Gamble Co., Cincinnati, Ohio J. F. MEAD, School of Medicine, University of California at Los Angeles, Los Angeles, California D. A. TURNER, Sinai Hospit...

Study of phenylketonuria by paper chromatography

The quantitative separation of estrogens by paper partition chromatography.

The varying success of known chromat’ographic procedures for the separation and identification of microquantities of estrogens (1, 2) has led to the development of paper partit!ion chromatographic techniques which are reported here. These t’echniques permit rapid separation, identification, and quantitative estimation of free est.rogens in biological materials. The solvent systems of o-dichloro...